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Image Search Results
Journal: PLoS ONE
Article Title: Differentially Expressed Plasma MicroRNAs and the Potential Regulatory Function of Let-7b in Chronic Thromboembolic Pulmonary Hypertension
doi: 10.1371/journal.pone.0101055
Figure Lengend Snippet: Human PAECs or PASMCs were transfected with a let-7b antagonist lentivirus or let-7b mimics, and the migration was evaluated by wound healing assays. Pictures were taken at 0 h, 12 h, 24 h, and 48 h. The wounded area was expressed as the percentage of recovery. ( A ) Let-7b mimics suppressed PAECs migration, and its antagonist promoted TGF-β induced PAECs migration at 24 h (n = 5). ( B ).Let-7b mimics suppressed PASMCs migration, and its antagonist promoted TGF-β induced PASMCs migration at 24 h (n = 5). P value was calculated by two-way ANOVA, and Post Hoc Test was done by Student-Newman-Keuls method. *** P <0.001. * P <0.05.
Article Snippet: Migration of human PAECs and
Techniques: Transfection, Migration
Journal: PLoS ONE
Article Title: Proteomic Analysis Reveals that Proteasome Subunit Beta 6 Is Involved in Hypoxia-Induced Pulmonary Vascular Remodeling in Rats
doi: 10.1371/journal.pone.0067942
Figure Lengend Snippet: A and B : The effects of PSMB6 siRNA (siPSMB6) on PSMB6 protein expression in PASMCs. PASMCs were transfected with 25 nM of non-targeting control siRNA (siNT) or siPSMB6 for 6 h in serum-free SMBM. PASMCs were then exposed to siRNA for 72 h before subsequent analysis with Western blotting. C : Effects of prolonged hypoxia and siPSMB6 on the proliferation of PASMCs by BrdU assay. PASMCs were treated with normoxia or prolonged hypoxia (4% O 2 , 60 h) with or without siPSMB6 treatment at dosages of 25 nM. Bar values are means ± SEM. * P <0.05 compared with normoxia nontargeting siRNA. § P <0.05 compared with hypoxic nontargeting siRNA ( n = 3 in each group).
Article Snippet:
Techniques: Expressing, Transfection, Control, Western Blot, BrdU Staining